This directory contains alignments of the following assemblies: - target/reference: Mouse (mm6, March 2005, NCBI Build 34) - query: Human (hg16, July 2003, NCBI Build 34) Files included in this directory: - md5sum.txt: md5sum checksums for the files in this directory - mm6.hg16.all.chain.gz: chained blastz alignments. The chain format is described in http://genome.ucsc.edu/goldenPath/help/chain.html . - mm6.hg16.net.gz: "net" file that describes rearrangements between the species and the best Human match to any part of the Mouse genome. The net format is described in http://genome.ucsc.edu/goldenPath/help/net.html . - axtNet/*.mm6.hg16.net.axt.gz: chained and netted alignments, i.e. the best chains in the Mouse genome, with gaps in the best chains filled in by next-best chains where possible. The axt format is described in http://genome.ucsc.edu/goldenPath/help/axt.html . - mm6.hg16.over.chain.gz: chained and netted alignments in chain format. The mm6 and hg16 assemblies were aligned by the blastz alignment program, which is available from Webb Miller's lab at Penn State University (http://www.bx.psu.edu/miller_lab/). Any mm6 sequences larger than 10,010,000 bases were split into chunks of 10,010,000 bases overlapping by 10,000 bases for alignment. A similar process was followed for hg16, with chunks of 30,000,000 overlapping by 0.) Following alignment, the coordinates of the chunk alignments were corrected by the blastz-normalizeLav script written by Scott Schwartz of Penn State. Transposons that have been inserted since the Human/Mouse split were removed from the assemblies before alignment using the fasta-subseq and strip_rpts programs from Penn State. The abbreviated genomes were aligned with blastz, and the transposons were then added back in (i.e. the alignment coordinates were adjusted) using the restore_rpts program from Penn State. The blastz scoring matrix (Q parameter) used was the default matrix: A C G T A 91 -114 -31 -123 C -114 100 -125 -31 G -31 -125 100 -114 T -123 -31 -114 91 with a gap open penalty of O=400 and a gap extension penalty of E=30. The minimum score for an alignment to be kept was K=3000 for the first pass and L=2200 for the second pass, which restricted the search space to the regions between two alignments found in the first pass. The minimum score for alignments to be interpolated between was H=2000. The .lav format blastz output was translated to the .psl format with lavToPsl, then chained by the axtChain program. Chained alignments were processed into nets by the chainNet, netSyntenic, and netClass programs. Best chains (.over.chain) were extracted from the nets and the set of all chained alignments by the netChainSubset program. Best-chain alignments in axt format were extracted by the netToAxt program. All programs run after blastz were written by Jim Kent at UCSC. ---------------------------------------------------------------- If you plan to download a large file or multiple files from this directory, we recommend you use ftp rather than downloading the files via our website. To do so, ftp to hgdownload.cse.ucsc.edu, then go to the directory goldenPath/mm6/vsHg16/. To download multiple files, use the "mget" command: mget <filename1> <filename2> ... - or - mget -a (to download all files in the current directory) All files in this directory are freely available for public use. -------------------------------------------------------------------- References Chiaromonte, F., Yap, V.B., and Miller, W. Scoring pairwise genomic sequence alignments. Pac Symp Biocomput 2002, 115-26 (2002). Kent, W.J., Baertsch, R., Hinrichs, A., Miller, W., and Haussler, D. Evolution's cauldron: Duplication, deletion, and rearrangement in the mouse and human genomes. Proc Natl Acad Sci USA 100(20), 11484-11489 (2003). Schwartz, S., Kent, W.J., Smit, A., Zhang, Z., Baertsch, R., Hardison, R., Haussler, D., and Miller, W. Human-mouse alignments with BLASTZ</A>. Genome Res. 13(1), 103-7 (2003).
Name Last modified Size Description
Parent Directory - axtNet/ 2005-04-28 00:10 - md5sum.txt 2005-04-28 00:10 2.9K mm6.hg16.net.gz 2005-04-28 00:01 126M mm6.hg16.all.chain.gz 2005-04-27 22:43 246M