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This project is in collaboration with the Human Genome Project,
directed by Francis Collins.
All human genome sequence data used has been generated by the laboratories
involved the human genome sequencing consortium, listed below.
Additional sources of data and genome annotation are given at
NHGRI's Genome Hub,
Human Genome Central,
EBI, and
Human Genome Central, NCBI.
The working draft sequence and its annotation are described in the paper
Initial Sequencing and
Analysis of the Human Genome by the International Human Genome Sequencing
Consortium, Nature, 409: 860-921, Feb. 2001.
The following specific resources were used by this project:
- A
fingerprint-based map of BAC clones fro the human genome
primarily from the RP-11 library,
and a corresponding layout of sequenced clones,
prepared under the direction of Robert
Waterston at
Washington University in St. Louis.
- A set of processed, managed DNA sequences from this layout, split at
the appropriate point for working draft clones and with obvious
contaminants removed, prepared by Greg Schuler at NCBI, taken from
public sequence database submissions. Also, specially formated versions of the finished chromosomes
21 and 22 from Greg Schuler, prepared from data generated at
the centers that sequenced these chromosomes. See
the sites at the
Max Plank Institute and
RIKEN for more information
on Chromosome 21, and the
Sanger Centre. for more information on
Chromosome 22.
- EST, mRNA and BAC end sequence data and information taken
from GenBank.
- In the September 5 freeze, we used plasmid end reads generated by
the Whitehead Institute for
Biomedical Research for the
SNP consortium.
In October we also used plasmid end reads from the
Washington University Genome
Sequencing Center and the
Sanger Centre generated for the
SNP consortium.
- In the browser for the July 17 freeze, gene predictions were supplied by
David Kulp and Alan Williams from Affymetrix
and Ewan Birney from Ensembl.
David Kulp and Alan Williams also supplied predictions for known genes processed through Genie.
The CpG island track was supplied by Kim Worley and John Bouck, and the
simple repeats track by
James Durbin at
Baylor College of Medicine.
The duplications track data was provided by Evan Eichler and Jeff Bailey
at Case Western Reserve University.
Lukas Wagner and Greg Schuler at NCBI supplied the data for the 3' EST track,
which we filtered further before displaying.
- Along with updated tracks from the July 17 freeze browser,
in the browser for the Sept. 5 freeze
the SNPs tracks were provided by
Lincoln Stein and the SNP consortium.
Greg Schuler provided collections of map data used in the STS track
from the following original sources:
Genethon genetic map,
Marshfield genetic
map,
GeneMap99 radiation hybrid
map,
G3
radiation hybrid map, and the
Whitehead YAC
map. David Cox provided STS markers for the
TNG radiation hybrid map.
We placed the above STS markers on the genome sequence using a very slightly
modified version of Schuler's e-PCR program.
Cytogenetic markers (FISH-mapped clones) were provided by Barbara Trask
and were generated by the BAC Resource
Consortium, as described more fully in
"Integration of cytogenetic landmarks into the draft sequence of the
human genome,"
by the BAC Resource Consortium, Nature, Vol. 409.
These markers were placed on the draft sequence by Wonhee Jang of NCBI.
Sean Eddy at Wash. U.
provided the RNA genes track.
Olivier Jaillon at Genoscope provided the Exofish track.
Further
Exofish tools and results are described in
'Estimate of human gene number provided by genome-wide analysis using Tetraodon
nigroviridis DNA sequence' Nature Genetics volume 25 page 235, June 2000.
Data for the mouse synteny track was provided by Deanna Church at NCBI. More
details can be found at the
NCBI human-mouse homology map.
LaDeana Hillier at Wash. U. provided an additional CpG islands track.
- Updated versions of most of the tracks from the Sept. 5 freeze browser
were provided by the same groups for the Oct. 7 freeze browser.
The Fgenesh++ gene predictions were produced by Softberry Inc.
See the paper "Ab initio gene finding in
Drosophila genomic DNA", Genome Research 10(5)
516-522 for more information on the method.
The data for the exonerate mouse track was kindly provided by
Guy Slater, Michele Clamp,
and Ewan Birney from Ensembl.
- In the Dec. 12 freeze browser, the STS markers and FISH-mapped
clones were placed by Terry Furey at UCSC. He also did the cytogenetic bands
in this and earlier browsers.
The following resources use the information provided by this project:
- The gene annotations and genome browser, providing multiple views of
a variety of key genome annotations, built
by the Ensembl group at EBI and the Sanger Centre.
A closely related set of resources:
- A separate assembly and annotations from
NCBI.
The institutions that form the Human Genome Sequencing Consortium include:
- Baylor College of Medicine,
Houston, Texas, USA
- Beijing Human Genome Center, Institute
of Genetics, Chinese Academy of Sciences, Beijing, China
- Cold Spring Harbor Laboratory,
Lita Annenberg Hazen Genome Center, Cold Spring Harbor, NY, USA
- Gesellschaft für Biotechnologische
Forschung mbH, Braunschweig, Germany
- Genoscope, Evry, France
- Genome Therapeutics Corporation,
Waltham, MA, USA
- Institute for Molecular
Biotechnology, Jena, Germany
- Joint Genome Institute, U.S.
Department of Energy, Walnut Creek, CA, USA
- Keio University, Tokyo,
Japan
- Max Planck Institute
for Molecular Genetics, Berlin, Germany
- RIKEN Genomic Sciences Center,
Saitama, Japan
- The Sanger Centre, Hinxton,
U.K.
- Stanford Genome Technology Center,
Palo Alto, CA, USA
- Stanford Human Genome Center,
Palo Alto, CA, USA
- University of
Oklahoma's Advanced Center for Genome Technology, OK, USA
- University of
Texas Southwestern Medical Center at Dallas, TX, USA
- University of
Washington Genome Center, Seattle, WA, USA
-
Multimegabase Sequencing Center, Institute for Systems Biology, Seattle, WA,USA
- Whitehead Institute for
Biomedical Research, MIT, Cambridge, MA, USA
- Washington University Genome
Sequencing Center, St. Louis, MO, USA
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