This directory contains alignments of the D. pseudoobscura assembly (dp2, Aug. 2003, BCM HGSC Freeze 1) to the D. melanogaster assembly (dm1, Jan. 2003, BDGP Release 3.1). Files included in this directory: - axtNet directory: contains chained and netted alignments, i.e. the best chains in the genome, with gaps in the best chains filled in by next-best chains where possible. - pseudoobscura.chain.zip: chained blastz alignments. The chain format is described in http://genome.ucsc.edu/goldenPath/help/chain.html. - pseudoobscura.net.zip: "net" file that describes rearrangements between the species and the best D. pseudoobscura match to any part of the D. melanogaster genome. The net format is described in http://genome.ucsc.edu/goldenPath/help/net.html. - md5sum.txt: checksums of the files in this directory The alignments in the axtNet directory are in "axt" format. For a description, see http://genome.ucsc.edu/goldenPath/help/axt.html. The alignments were produced by the blastz alignment program, which is available from Webb Miller's lab at Penn State University (http://www.bx.psu.edu/miller_lab/). The blastz scoring matrix used was: A C G T A 91 -114 -31 -123 C -114 100 -125 -31 G -31 -125 100 -114 T -123 -31 -114 91 with a gap open penalty of 400 and a gap extension penalty of 30. The minimum score for an alignment to be kept was 3000 for the first pass and 2200 for the second pass, which restricted the search space to the regions between two alignments found in the first pass. Each chromosome was divided into 10,010,000 base chunks with 10,000 bases of overlap. The .lav format blastz output, which does not include the sequence, was converted to .axt with lavToAxt. The axtNet alignments were processed with chainNet, netSyntenic, and netClass written by Jim Kent at UCSC. --------------------------------------------------------------- If you plan to download a large file or multiple files from this directory, we recommend that you use ftp rather than downloading the files via our website. To do so, ftp to hgdownload.cse.ucsc.edu, then go to the directory goldenPath/dm1/vsDp2/. To download multiple files, use the "mget" command: mget <filename1> <filename2> ... - or - mget -a (to download all the files in the directory) All the files in this directory are freely available for public use.
Name Last modified Size Description
Parent Directory - axtNet/ 2004-08-17 11:58 - md5sum.txt 2004-08-17 11:58 703 pseudoobscura.chain.gz 2004-08-17 11:55 10M pseudoobscura.net.gz 2004-08-17 11:55 10M