This directory contains the June 2007 assembly of the marmoset genome
(calJac1, WUSTL Callithrix_jacchus-2.0.2), as well as repeat annotations 
and GenBank sequences.

This assembly was produced by the Genome Sequencing Center at the
Washington University School of Medicine in St. Louis.
For more information on the marmoset genome, see the project website:
http://genome.wustl.edu/genome.cgi?GENOME=Callithrix%20jacchus

Files included in this directory:

calJac1.2bit - contains the complete marmoset/calJac1 genome sequence
    in the 2bit file format.  Repeats from RepeatMasker and Tandem Repeats
    Finder (with period of 12 or less) are shown in lower case; non-repeating
    sequence is shown in upper case.  The utility program, twoBitToFa (available
    from the kent src tree), can be used to extract .fa file(s) from
    this file.  A pre-compiled version of the command line tool can be
    found at:
        http://hgdownload.cse.ucsc.edu/admin/exe/linux.x86_64/
    See also:
        http://genome.ucsc.edu/admin/git.html
	http://genome.ucsc.edu/admin/jk-install.html

calJac1.agp.gz - Description of how the assembly was generated from
    fragments.

calJac1.fa.gz - "Soft-masked" assembly sequence in one file.
    Repeats from RepeatMasker and Tandem Repeats Finder (with period
    of 12 or less) are shown in lower case; non-repeating sequence is
    shown in upper case.

calJac1.fa.masked.gz - "Hard-masked" assembly sequence in one file.
    Repeats are masked by capital Ns; non-repeating sequence is shown in
    upper case.

calJac1.fa.out.gz - RepeatMasker .out file.  RepeatMasker was run with the
    -s (sensitive) setting.  Repeat Masker version:
	#    May 17 2007 (open-3-1-8) version of RepeatMasker
    Repeat Masker library version: RELEASE 20061006; 

calJac1.trf.bed.gz - Tandem Repeats Finder locations, filtered to keep repeats
    with period less than or equal to 12, and translated into UCSC's BED
    format.

est.fa.gz - Marmoset ESTs in GenBank. This sequence data is updated once a
    week via automatic GenBank updates.

calJac1.quals.fa.gz - quality scores in fasta file format

md5sum.txt - checksums of files in this directory

mrna.fa.gz - Marmoset mRNA from GenBank. This sequence data is updated
    once a week via automatic GenBank updates.

upstream1000.fa.gz - Sequences 1000 bases upstream of annotated
    transcription starts for Xeno RefSeq genes with annotated 5' UTRs.  
    This file is updated weekly so it could be slightly out
    of sync with the Xeno RefSeq data which is updated daily for most 
    assemblies.

upstream2000.fa.gz - Same as upstream1000, but 2000 bases.

upstream5000.fa.gz - Same as upstream1000, but 5000 bases.

xenoMrna.fa.gz - GenBank mRNAs from species other than that of 
    the genome. This sequence data is updated once a week via automatic 
    GenBank updates.


calJac1.chrom.sizes - Two-column tab-separated text file containing assembly
    sequence names and sizes.

------------------------------------------------------------------
If you plan to download a large file or multiple files from this 
directory, we recommend that you use ftp rather than downloading the 
files via our website. To do so, ftp to hgdownload.cse.ucsc.edu, then 
go to the directory goldenPath/calJac1/bigZips. To download multiple 
files, use the "mget" command:

    mget <filename1> <filename2> ...
    - or -
    mget -a (to download all the files in the directory)

Alternate methods to ftp access.

Using an rsync command to download the entire directory:
    rsync -avzP rsync://hgdownload.cse.ucsc.edu/goldenPath/calJac1/bigZips/ .
For a single file, e.g. chromFa.tar.gz
    rsync -avzP 
        rsync://hgdownload.cse.ucsc.edu/goldenPath/calJac1/bigZips/chromFa.tar.gz .

Or with wget, all files:
    wget --timestamping 
        'ftp://hgdownload.cse.ucsc.edu/goldenPath/calJac1/bigZips/*'
With wget, a single file:
    wget --timestamping 
        'ftp://hgdownload.cse.ucsc.edu/goldenPath/calJac1/bigZips/chromFa.tar.gz' 
        -O chromFa.tar.gz

To unpack the *.tar.gz files:
    tar xvzf <file>.tar.gz
To uncompress the fa.gz files:
    gunzip <file>.fa.gz

------------------------------------------------------------------
The Marmoset sequence is made freely available to the community by the
Genome Sequencing Center, Washington University School of Medicine, with
the following understanding:

1. The data may be freely downloaded, used in analyses, and repackaged in
   databases.

2. Users are free to use the data in scientific papers analyzing these data
   if the providers of these data are properly acknowledged.  See
   http://genome.ucsc.edu/goldenPath/credits.html for credit information.

   It is requested that users of this marmoset sequence assembly acknowledge
   the Washington University School of Medicine, Genome Sequencing Center
   in any publications that result from use of this sequence assembly.

3. The centers producing the data reserve the right to publish the initial
   large-scale analyses of the data set, including large-scale identification
   of regions of evolutionary conservation and large-scale genomic assembly.
   Large-scale refers to regions with size on the order of a chromosome (that
   is, 30 Mb or more).

4. Any redistribution of the data should carry this notice.
      Name                    Last modified      Size  Description
Parent Directory - calJac1.chrom.sizes 2007-08-21 14:54 845K calJac1.2bit 2007-09-05 14:44 757M calJac1.agp.gz 2008-01-11 09:59 6.1M calJac1.fa.out.gz 2008-01-11 10:00 158M calJac1.trf.bed.gz 2008-01-11 10:00 5.6M calJac1.fa.gz 2008-01-11 10:14 912M calJac1.fa.masked.gz 2008-01-11 10:23 505M calJac1.quals.fa.gz 2009-06-08 14:07 535M md5sum.txt 2014-01-03 15:02 358 xenoMrna.fa.gz 2016-03-22 20:45 5.0G xenoMrna.fa.gz.md5 2016-03-22 20:46 49 mrna.fa.gz 2019-10-16 19:54 347K mrna.fa.gz.md5 2019-10-16 19:54 45 est.fa.gz 2019-10-16 19:58 63M est.fa.gz.md5 2019-10-16 19:58 44 xenoRefMrna.fa.gz 2019-10-16 19:59 331M xenoRefMrna.fa.gz.md5 2019-10-16 19:59 52 refMrna.fa.gz 2019-10-16 19:59 165K refMrna.fa.gz.md5 2019-10-16 19:59 48 upstream1000.fa.gz 2019-10-16 20:00 16M upstream1000.fa.gz.md5 2019-10-16 20:00 53 upstream2000.fa.gz 2019-10-16 20:01 31M upstream2000.fa.gz.md5 2019-10-16 20:01 53 upstream5000.fa.gz 2019-10-16 20:02 86M upstream5000.fa.gz.md5 2019-10-16 20:02 53 genes/ 2020-02-05 13:46 -