This track displays FPKM (fragments per kilobase of exon per million mapped fragments) gene expression values from a mouse developmental time course bulk RNA-seq experiment covering 17 tissues across up to 8 time points (embryonic days 10.5 through 16.5 and postnatal day 0). Values were generated by RSEM from alignments to the mm10 reference genome using the GENCODE M21 annotation set. Each bar in the chart represents one replicate from one tissue at one developmental time point.
The data were produced as part of the ENCODE project at the Wold Lab, Caltech. Each tissue and time point has two biological replicates, each displayed as its own bar.
This track is displayed as a bigBarChart. Bars are colored by tissue type and can be faceted by tissue and timepoint using the track configuration controls.
Bars are colored by tissue:
| Color | Tissue |
|---|---|
| adrenal gland | |
| embryonic facial prominence | |
| forebrain | |
| heart | |
| hindbrain | |
| intestine | |
| kidney | |
| limb | |
| liver | |
| lung | |
| midbrain | |
| neural tube | |
| skeletal muscle tissue | |
| spleen | |
| stomach | |
| thymus | |
| urinary bladder |
Within each tissue, bars are grouped by developmental time point: e10.5, e11.5, e12.5, e13.5, e14.5, e15.5, e16.5, and P0. Not all tissues are available at all time points.
RNA-seq reads were processed using the ENCODE long RNA-seq pipeline. Reads were aligned to the mm10 genome using STAR (Dobin et al., 2013) and quantified at the gene level using RSEM (Li and Dewey, 2011) with the GENCODE M21 annotation set. The FPKM column from the RSEM gene quantification files across all tissues and replicates was concatenated into a single matrix. This matrix was then converted into the UCSC faceted bigBarChart format (bed, categories, and facet files), and the bed file was converted into a bigBarChart bigBed file.
The data in this track can be explored interactively with the Table Browser or the Data Integrator. For programmatic access, the track can be accessed using the Genome Browser's REST API.
The bigBarChart bigBed file underlying this track can be downloaded from our download server. Individual regions or the whole genome annotation can be obtained using our tool bigBedToBed, which can be compiled from the source code or downloaded as a precompiled binary for your system. Instructions for downloading source code and binaries can be found here.
The original data files and experimental metadata are available from the ENCODE Portal.
Please refer to our Data Access FAQ for more information.
Peng He (Peng.He@ucsf.edu) was the lead author for this project and performed the data analysis. Diane Trout (diane@caltech.edu) at the Wold Lab, Caltech, was responsible for data management. The data were produced as part of the ENCODE Consortium.
Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR. STAR: ultrafast universal RNA-seq aligner. Bioinformatics. 2013 Jan 1;29(1):15-21. PMID: 23104886; PMC: PMC3530905
Li B, Dewey CN. RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinformatics. 2011 Aug 4;12:323. PMID: 21816040; PMC: PMC3163565